汕头地区食管癌人乳头状瘤病毒的原位杂交检测

Detection of Human Papillomavirus (HPV) on Esophageal carcinoma in shantou District by IN-Situ-Hybridization

５５例新鲜食管癌冰冻切片用ＩＳＨ检测ＨＰＶ感染状况，４５例阳性，阳性率８１．８％。不同病变ＨＰＶ感染率不同，正常（８７．５０％）和单纯增生（８１．４０％）感染率明显高于原位癌（３８．３９％）和浸润癌（２７．２７％），不典型增生居中（６４．７０％）；而且不同病变ＩＳＨ阳性信号分布特征不同，在正常和单纯增生上皮呈层状分布，表层阳性细胞数多，信号强；原位癌层状分布特征消失，较弥散，浸润癌呈区域性阳性。本实验表明汕头食管癌组织ＨＰＶ感染率高，ＨＰＶ１８可能在该地区食管癌的发病过程中起重要作用。

cases of frozen sections from fresh esophageal carcinoma were detected for HPV by ISH.45/55cases were HPV positive. The infection rate varied from lesion to lesion:normal mucosa(87.50%)and simple hyperplasia epithelia(81.40%)were higher than that of cancer in-situ(38. 39%)and invasive cancer (27. 27%).Dysplasia (64.70%) was the intermediate ones. More over, HPV hybridization signals were also related to the epithelial lesions. The positive spots were distributed in superficial layers in normal and simple hyperplasitic epithelia.The closer to the epithelial surface,the more postive cells and stronger signals were observed.Hybridization signals were diffusely distributed and the 'layer feature' disappeared in carcinoma in-situ.The spots were focolly positive in invasive cancer.Our deta showed a high HPV infection rate in esophageal cancer in shantou District.HPV 18 might play an important role in the pathogenesis of this malignancy in this area.