摘要
高效表达外源蛋白 ,在理论和实践上特别是在生物制药中具有重要意义 ,巴氏毕赤酵母 (Pichiapastoris)是表达外源蛋白最理想的真核表达系统之一 .影响外源蛋白在P .pastoris中表达的因素很多 ,主要包括外源基因自身的特性、载体、宿主细胞几个方面 ,了解和灵活运用它们的联系 ,有助于获得外源基因在P .
It is very important to produce heterologous proteins both in theory and in practice,expecially for biopharmaceutical.Pichia pastoris has become one of the best eucaryotic expression systems in recent years.The factors influencing the high-level expression of heterologous protein in P.pastoris and the core strategies to hyperexpress heterologous proteins in P.pastoris were discussed in this paper.
基金
国家自然科学基金资助项目 ( 3 9670 3 92 )
