乳腺癌CD44和nm23基因表达与侵袭转移的关系

CD44 AND nm23 EXPRESSION IN BREAST CARCINOMA AND THEIR RELATION TO TUMOR INVASION AND METASTASIS

目的 :探讨粘附分子 CD44和抑癌基因 nm2 3与乳腺癌侵袭转移的关系。方法 :采用逆转录聚合酶链反应 (RT- PCR)方法对 30例乳腺癌及 10例乳腺良性肿瘤组织中 CD44 V6和 nm2 3- H1基因的表达进行检测。结果 :乳腺癌组织中 CD44 V6及nm2 3- H1的表达明显高于乳腺良性肿瘤 ;CD44 V6及 nm2 3- H1的阳性表达与乳腺癌的临床分期和是否有淋巴结转移密切相关 ,而与乳腺癌的病理类型无关。结论 :CD44 V6和 nm2 3- H1异常表达是乳腺癌发生发展、浸润转移的重要分子学改变 ;同时检测两者可更好地预测其进展程度和对淋巴结转移的判断。

Objective: To explore the the expressions of CD44V6 and nm23 in breast carcinoma and their relation to tumor invasion and metastasis. Methods: The CD44V6 and nm23-H1 gene products were detected in 30 breast cancer specimens and 10 benign breast tumor tissues by reverse transcription polymerase chain reaction (RT-PCR). Results: The expressions of CD44V6 and nm23-H1 in breast carcinoma were markly higher than that in benign tumors. CD44V6 and nm23-H1 expressions of breast carcinoma tissues were significantly correlated with TNM clinical stages and metastasis to lymph nodes. However, the expressions of CD44V6 and nm23-H1 were not related with pathological type of tumor. Conclusion: The abnormal expressions of CD44V6 and nm23-H1 might be involved in the progression, invasion and metastasis of breast carcinoma. Combined assay of CD44V6 and nm23-H1 expressions could be useful in assessing tumor progression and lymphatic metastasis of breast carcinoma.