红麻微生物脱胶菌种的选育

Screen of microbial strain for bluish dogbne degumming

从红麻生产地土壤与沤麻废水中 ,分离出多株产果胶酶的菌株。经筛选 ,复筛获得了 5株厌氧细菌 ;并选其中在果胶平板上生长速度快、透明圈大、脱胶效果好的一株DQ1,作为生物脱胶的目的菌。初步鉴定为厌氧芽孢杆菌。找到了最适培养条件 :最适氮源为复合有机氮 ,用量 1.5 %最适碳源为果胶 ,用量 0 .4%最适温度 30～ 35℃ ,pH 7.0～ 7.5 ,接种量 3 % ,培养时间 2 0h .用于红麻脱胶实验 ,比对照天然水沤麻脱胶 ,周期可缩短 30 %左右 .红麻纤维柔软度、断裂强力。

We have separated several kinds of pectinase producing strain from bluish dogbane planting soil and its retting effluent, and find five anaerobic bacterias through screening. DQ 1 is the quickest growing strain with larger transparent circle on pectin plate media and stronger capacity for degumming. It is identified as anaerobic bacillus. We find the following optimal cultivation: the media containing 1.5% of complexed organic nitrogen and 0.4% pectin, initial pH value 7.0-7.5, cultivating with 3% inoculum and at 30-35℃ for 20 hours. The period of time is reduced by 30%, comparing with that degumming in natural water. The softness, break strength, and line density of the bluish dogbane are improved significantly.