慢性肝病时肝内谷胱甘肽S-转移酶的免疫组织化学观察

Immunohistochemical Study of Glutathione S-Transferases in Chronic Liver Diseases

用兔抗人总谷胱甘肽转移酶(GSTs)抗体在健康人、慢性乙型肝炎、肝硬化及肝癌患者的肝组织石蜡切片上,以 ABC 法进行免疫组化检测。健康人肝组织 GSTs 阳性主要出现在肝细胞核(5/15) ,慢性肝病患者 GSTs 胞浆阳性率显著增高(p<0. 001) ,阳性程度亦较强。肝细胞膜 GSTs 在健康人未发现,CPH 为6. 2％(1/16) ,CAH 为25％(5/20) ,肝硬化合并肝癌者80％(12/15) ,随着病变进展而明显增加。肝硬化时增生活跃的肝细胞和胆小管上皮细胞 GSTs 亦常为阳性。

An immunohistochemical assay for GSTs with rabbit anti-GSTs antibody in fo- rmalin-fixed,paraffin embedded liver sections from healthy individuals and chronic hepatitis B,cirrhosis,or hepatocellular carcinoma patients was performed. In healthy casess GSTs were possitive mainly in nuclei (5/5) ,whereas in chronic liver diseases,GSTs possitive rate in hepatocyte cytoplasms increased signifi- cantly (p<0. 001) .No GSTs on cell membrane were found in healthy liver tissue, but 6. 2%(1/16) in CPH,25%(5/20) in CAH,80%(12/15) in cirrhosis with or with- out HCC.The results showed that GSTs on cell membrane increased as the liver lesions progressed.