摘要
用光镜、电镜和体视学方法观察和分析经不同抗原免疫接种兔被蜱叮咬的局部组织病理变化,以了解局部组织的反应性。结果表明佐剂对照组、纯化抗原组和唾液腺抗原组兔被叮咬后均出现炎症变化,佐剂对照组和纯化抗原组反应较轻,唾液腺抗原组反应较重;佐剂对照组主要为中性粒细胞浸润;免疫接种组主要为嗜碱性细胞浸润,并可见脱颗粒现象。局部组织细胞数密度纯化抗原组显著低于唾液腺抗原组,提示纯化抗原接种既可诱导宿主产生较强的获得性免疫力,局部反应又较轻,是抗蜱疫苗主要候选抗原之一。
The histopathological changes in skin of host rabbits vaccinated with antigen when they were infested by ixodes sinensis were studied with light microscope,electronic microscope and stereological methods for analyzing the local response.The results showed that the in- flammation occurred at rabbits after being infested in all 3 groups,adjuvant control group (AC),salivary gland antigen group(SGA)and the 105 KD purified whole tick antigen group (PA)and light in AC groupbut heavy in PA group.The inflammation cells were mainly neu- trophil in AC group while mainly basophil in SGA and PA groups and their threshing appear- ance could been seen.The cellular numeric density in inflammation tissue in PA group was much lower than in SGA group.The fact that when hos was vaccinated with 105 KD purified whole tick antigen not only could produce resistance to tick but also was light local response suggested that it may serve as an effective antigen for the choice of anti-tick vaccine.
出处
《江西医学院学报》
1998年第4期1-4,F004,共5页
Acta Academiae Medicinae Jiangxi
基金
国家自然科学基金(39660073)
江西省自然科学基金(964028)
