丙型肝炎病毒基因NS_5区酶切分型研究

Genotyping of NS_5 Region of HCV by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism Analysis

目的 :了解丙型肝炎病毒 (HCV)感染的基因型及其与临床的关系。方法 :用逆转录套式聚合酶链反应 (RT PCR)和限制性片断长度多态性 (RFLP)分析的方法 ,对 78例HCV感染患者进行HCVNS5区基因分型。结果 :HCV 1b型感染 71例 (91% ) ,HCV 2a型 7例 (9% ) ,未发现其它基因型 ;丙型肝炎患者有无输血史、是否重叠HBV感染 ,其基因分型构成比均无显著性差异 (P >0 .0 5 ) ;1b型HCV血清ALT异常者 (6 3.4% )明显多于 2a型(14.3 % ) (P <0 .0 5 )。结论 :本组HCV感染的基因型多数为 1b型 ,少数为 2a型 ,1b型更易致肝细胞损伤 。

Objective: To study the genotypes of HCV and their relationship to clinical profiles. Methods: Genotyping of NS 5 region of HCV genome was carried out in 78 HCV_infected patients by reverse transcription nested polymerase chain reaction (RT_PCR) and restriction fragment length polymorphism (RFLP) analysis. Results: Seventy one patients (91%) were infected with HCV type 1b and 7 (9%) with type 2a, but not type 1a,2b or others. The distribution of HCV genotypes in the patients with transfusion history was similar to the ones without transfusion histroy (P>0.05),and only HCV infection was also similar to HCV and HBV co_infection (P>0.05). Abnormal serum ALT was found more in patients with type 1b than those with type 2a (P<0.05). Conclusion: The majority of hepatitis C patients were with HCV type 1b infection,and the minority were with type 2a HCV. Type 1b HCV can more easily lead to hepatocellular injury 2a,and may be related to active hepatitis.