膀胱肿瘤尿脱落细胞端粒酶活性分析

Telomerase Activity of Exfoliated Cells in Urine in Patients with Bladder Cancer

目的 :检测膀胱肿瘤患者尿脱落细胞端粒酶活性表达 ,探讨端粒酶活性对膀胱肿瘤早期诊断及术后监测的意义。方法 :采用基于PCR的端粒重复序列扩增 (TRAP)结合银染的方法 ,分别对自 1998年 1月至 1999年 6月收集的 2 5例膀胱肿瘤组织标本及尿脱落细胞标本 ,和 2 3例非膀胱肿瘤患者尿脱落细胞标本进行端粒酶活性检测。结果 :2 5例膀胱肿瘤组织及相应患者的自排脱落细胞端粒酶活性阳性率分别为 84%和 80 % ,而 2 3例非膀胱肿瘤患者尿脱落细胞仅一例表达有端粒酶活性 ,膀胱肿瘤患者与非肿瘤患者自排尿脱落细胞端粒酶活性表达比较差异有显著性 (P <0 .0 1) ,G1,G2 ,G3期膀胱肿瘤患者自排尿脱落细胞端粒酶活性阳性率分别为 71% ,70 % ,10 0 % ,G3期阳性率明显高于G1,G2期。结论

Objectives: To determine the telomerase activity of exfoliated cells in urine in patients with bladder cancer, and to evaluate its clinical significance for the early diagnosis and postoperative follow_up of bladder cancer. Methods: Telomerase activity was determined by a modified TRAP——silver stain assay in cancer tissue and exfoliated cells in urine from patients with bladder cancer ( n =25), and exfoliated cells in urine from patients without bladder cancer ( n =23). Results: The positive rate of telomerase activity was 84% in bladder cancer tissues; 80% in exfoliated cells from patients with bladder cancer (71% in period G1, 70% in period G2, 100% in period G3, respectively), 4%(1/23) in exfoliated cells from patients without bladder cancer. Conclusions: These results suggest that the determination of telomerase activity of exfoliated cells in urine in patients with bladder cancer, as a noninvasive method, could be utilized for the early diagnosis and postoperative follow_up of bladder cancer.