非离子型表面活性剂AE对大薸损伤程度的酶学诊断

ENZYME DIAGNOSING OF INJURED DEGREE OF PISTIA STRATIOTES L. BY NONIONIC SURFACTANT LINEAR ALKYLETHOXYLATE (AE)

以超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)和过氧化物酶 (POD)的比活力变化作为观测指标 ,进行了脂肪醇聚氧乙烯醚 (AE)对大薸 (PistiastratiotesL .)损伤程度的酶学诊断 .结果表明 :在 17℃下 ,ρ(AE) 0 .1mg L的AE对大薸的伤害程度极微 ;ρ(AE)为 1.0、10 .0mg L时 ,大薸受到较大的伤害 ,但能逐渐恢复正常生理活动 ;ρ(AE)为 2 0 .0、5 0 .0mg L时 ,由于伤害程度大 ,超出了酶的修复能力 ,组织逐渐坏死 .同时推测 :在AE污染下 ,大薸的POD是起保护作用的主导酶 .图 3表 1参

The injuried degree of Pistia stratiotes L. due to pollution of linear alkylethoxylate (AE) was diagnosed by the relative activities of three kinds of enzymes: surperoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). The results showed: Pistia stratiotes L. was injured slightly at ρ (AE) 0.1 mg/L at 17℃; when the concentration of AE was 1.0 or 10.0 mg/L, the injured degree increased, but Pistia stratiotes L. could recover because of these protection enzymes. When the concentration of AE was 20.0 or 50.0 mg/L, Pistia stratiotes L. was injured seriously, tissues were damaged gradually, the injury degree went beyond the ability of these enzymes. It was deduced that POD was the leading protection enzyme when Pistia stratiotes L. was in AE pollution environment. Fig 3, Tab 1, Ref 12