大熊猫乳汁蛋白组成

Protein Composition of the Giant Panda Milk

测定了 2只大熊猫 (Ailuropodamelanoleuca)乳汁中蛋白含量、乳糖含量、乳蛋白组成 ,并与成都麻羊 (Caprahircus)初乳和中国荷斯坦牛 (Bostaurus)乳进行了比较。大熊猫乳蛋白含量平均为 4 1 5 2g/L ,与中国荷斯坦牛乳接近 ;乳糖平均含量为 15 4 1g/L ,极显著低于牛乳和成都麻羊初乳。乳蛋白SDS PAGE分析表明 ,大熊猫乳中酪蛋白 (CN)含量明显低于羊乳和牛乳 ;乳中存在 3条蛋白含量高的区带 ,而在成都麻羊和中国荷斯坦牛乳电泳图谱的相应位置未见明显的区带 ;乳中上皮粘蛋白MUC1仅存在 1条分子量约为 196kDa的区带 。

Protein and lactose concentrations,protein composition were assayed in milk samples from two giant panda individuals.The results were compared with that of Chengdu Ma goat and Chinese Holstein.The average protein concentration in giant panda milk was 41 52?g/L,similar to Chinese Holstein milk.The average lactose content was 15 41?g/L,significantly lower than goat colostrum and bovine milk.The SDS PAGE profile of skim milk proteins of the giant pandas was different from that of bovine and goat.Casein content in the giant panda milk was lower than that of goat colostrum and bovine milk.Three protein bands with high content were observed in the giant pandas but not in the Chengdu Ma goat or Chinese Holstein.No polymophism of the giant panda milk MUC1 was found in the two milk samples,and only one band with molecular weight of approximately 196?kDa was observed on the SDS gel.