摘要
利用基因重组技术 ,将葡激酶基因克隆到枯草芽孢杆菌 (B.subtilis)中 ,经过发酵培养 ,葡激酶被高效表达 ,并以可溶性活性状态分泌到胞外。本文报道利用 SP Sepherose和 S- 2 0 0 Sephacryl二步柱层析来纯化葡激酶 ,最终纯度在 98%以上 ,得率为 5 0 %～ 6 0 %。
Staphylokinase gene was cloned in B.subtilis. It has been expressed highly under the desired fermentative conditions.Recombinant B.subtilis produced extracellular staphylokinase in a soluble,active form.Gram quantities of highly purified recombinant staphylokinase were obtained from fermentation broth by chromatography,on sp Sepharose and on s 200 Sephacryl columns,with yields of 50 to 60 percent and 98 percent purity.
出处
《天然产物研究与开发》
CAS
CSCD
2001年第3期39-42,共4页
Natural Product Research and Development
基金
福建省计划委员会科技攻关项目资助 ( 970 0 9)&&
关键词
重组葡激酶
细菌发酵
纯化
Recombinant staphylokinase
Bacterial fermentation
purification
