CTLA4-Ig影响T细胞功能及机理的初步研究

The preliminary study on the effect of CTLA4-Ig on T cell function and its mechanism

目的 :研究CTLA4 Ig融合蛋白对外周血T淋巴细胞活化的抑制作用及机理。方法 :分离新鲜人外周血单个核细胞 ,观察CTLA4 Ig对淋巴细胞转化和混合淋巴细胞培养的作用 ,用流式细胞术观察CTLA4 Ig对T细胞表面CD2 5表达的影响 ,最后通过EMSA方法检测激活T细胞中核蛋白的变化。结果 :CTLA4 Ig能够抑制淋巴细胞转化和混合淋巴细胞反应 ,减低T细胞表面CD2 5的表达 ,抑制核内与IL 2表达相关的RE AP结合因子的活化。结论 :CTLA4 Ig通过多种信号途径对T细胞的活化起作用 ,其机制之一是抑制核内结合于IL 2增强子RE AP位点的核因子的活化。

Objective:To study the inhibited function of CTLA4 Ig on T cell activation and its mechanism Methods:After separating fresh PBMC,the effects of CTLA4 Ig on T lymphocyte transformation and mixed lymphocyte reaction were analyzed,and its inhibitory effect on the CD25 expression on T cell surface was observed At last,the changes of the nuclear protein in active T cell were analyzed by the EMSA method.Results:CTLA4 Ig has an inhibitory effect on the lymphocyte transformation,MLR and CD25 expression on T cell surface,and it can inhibite the activation of RE/AP binding factor related to IL 2 expression. Conclusion:CTLA4 Ig inhibit the activation of T cell by multipathway,one of the mechanism might be its inhibitory effect on the activation of RE/AP binding factor.