抗人肝再生增强因子抗体识别部位的确定

Identification of the antigenic determinants of human augmenter of liver regeneration (hALR) recognized by its monoclonal antibodies

目的 :确定抗人肝再生增强因子单克隆抗体所识别的抗原表位。方法 :采用蛋白质预测软件预测人肝再生增强因子的抗原决定簇 ,根据预测结果合成肽进行竞争ELISA验证单克隆抗体所识别部位。结果 :人肝再生增强因子的第 6～ 15 ,6 8～ 80以及 10 5～ 112位氨基酸残基是其抗原表位。结论 :采用计算机软件预测 ,并在此基础上经合成肽验证的方法是一种可靠的抗原表位确定方法。

Objective:Identification of the antigenic determinants of hALR.Methods:Theoretic antigenic determinants of hALR was predicted by using Hopp&Wodds method and and Goldkey software package.The four polypeptides according to the amino acid sequences of the predictive linear epitopes of hALR were synthesized and were used to analyze the antigenic determinants of hALR recognized by antibodies.Results:The polypeptides corresponding to residues 6～5,68～80 and 105～112 of hALR were its antigenic determinants.Conclusion:Prediction of the protein antigenic determinants by computer program and detection of them by competitive ELISA with synthesized polypeptides is a useful method of identification of the antigenic determinants.