系统性红斑狼疮患者外周血单个核细胞中IL-4和IL-13mRNA的表达

Expression of IL-4 and IL-13 mRNA in the peripheral blood mononuclear cells of the patients with systemic lupus erythematosus

目的 :探讨Th2型细胞因子在系统性红斑狼疮 (SLE)发病机制中的作用及其意义。方法 :应用逆转录 聚合酶链反应 (RT PCR)检测了 34例活动期SLE患者和 30例正常人外周血单个核细胞 (PBMC)中IL 4和IL 1 3mRNA的表达水平。结果 :活动期SLE患者IL 4和IL 1 3的阳性表达率与正常人对照组相比均无明显差异 ( P >0 0 5) ;活动期SLE患者PBMC中IL 4和IL 1 3mRNA的平均表达水平 ( 0 9386± 0 1 6 89,0 8983± 0 1 1 53)均明显高于正常人对照组 ( 0 54 94± 0 1 51 0 ,0 6 0 85±0 0 90 3) ,差异非常显著 (P <0 0 0 1 )。结论 :Th2型细胞因子IL 4和IL 1 3在SLE患者中呈高水平表达 ,这可能与SLE患者外周血T细胞高度活化、功能异常有关。

Objective:To investigate the roles of Th 2 cytokine expression in the pathogenesis of systemic lupus erythematosus and their significance Methods:Applying reverse transcription polymerase chain reaction (RT PCR) technique to semiquantitatively analyze IL 4 and IL 13 mRNA expression in the Peripheral Blood Mononuclear Cells (PBMC) from 34 active SLE patients and 30 healthy subjects Results: Compared with normal controls, there was no statistical significance in the positive rates of IL 4 and IL 13 expression (P>0 05, both) The mean levels of IL 4 and IL 13 mRNA expression in active SLE group were significantly increased, and there were markedly statistical differences between patients with active SLE and normal controls (P<0 001, all) Conclusion:There is a up regulated expression of Th 2 cytokines in SLE patients,which may play prominent roles in the pathogenesis of systemic lupus erythematosus