摘要
目的 建立番荔枝科植物中抗肿瘤活性成分番荔素的HPLC含量测定方法。方法 Squamostatin B (1) ,squamocin (2 )和annonin VI (3)为标准品。色谱柱 :反相C1 8柱 ;流动相 :甲醇 水 (90∶10 ) ;流速 :1 0mL·min- 1 ;检测波长 :2 2 0nm。结果 进样量在 2 3- 13 8μg有良好的线性关系。 (1) ,(2 )和 (3)的回收率分别为 10 0 3% ,10 0 3%和10 0 0 %。结论 本法快速、简便、灵敏和分离度好 ,适用于番荔枝科植物中抗肿瘤活性成分番荔素的含量测定。
AIM To develop a method for analysis of antitumor annonaceous acetogenins in Annonaceae plants by HPLC. METHODS Squamostatin-B (1), squamocin (2) and annonin-VI (3) were used as standard substances. Chromatography column was a Rp-18; the mobile phase was methanol-water (90∶10); the flow rate was 1 0 mL·min -1 and the detecting wavelength was 220 nm. RESULTS A linear range was obtained from 2 3 to 13 8 μg with a good correlation. The recoveries of (1), (2) and (3) were 100 3%, 100 3% and 100 0%, respectively. CONCLUSION This method was developed for the analysis of acetogenins by HPLC for the first time. The method is rapid, accurate and suitable for the analysis of the antitumor acetogenins in Annonaceae plants.
出处
《药学学报》
CAS
CSCD
北大核心
2001年第9期683-685,共3页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目 ( 2 95 72 0 87)
