大鼠气道炎症与支气管高反应性的关系及茶碱的抑制作用

Relationship between airway inflammation and bronchial hyperreactivity in rats and the effect of theophylline

目的 :分析致敏大鼠在抗原攻击后嗜酸粒细胞炎性浸润为特征的气道炎症与支气管高反应性的相关性 ,并观察不同剂量茶碱的作用。方法 :在卵白蛋白 (OA)致敏的大鼠 ,用 1%OA气雾攻击后 ,以计算机辅助技术测定气道管壁面积、支气管周围嗜酸粒细胞数量以及对乙酰甲胆碱 (MCh)的反应性。结果 :OA攻击可诱导气道炎症反应和支气管高反应性 ,两者间有显著正相关。口服 7d茶碱 ( 1- 12 5mg/kg ,bid)可减轻气道炎症 (细支气管壁肿胀和肺嗜酸粒细胞浸润 ) ,对支气管高反应性也有一定抑制作用。结论 :提示支气管高反应性与气道炎症有正相关 ,小剂量茶碱有抗气道过敏性炎症的作用。

AIM: To determine the relationship between antigen-induced airway inflammation characterized by pulmonary eosinophilia and bronchial hyperreactivity in rats, and to evaluate the effect of theophylline at different doses. METHODS: In ovalbumin (OA)-sensitized rats, bronchiole wall area, eosinophils around bronchi, and the responses to methacholine (MCh) aerosol were measured after 1% OA aerosol challenge with computer-assisted techniques. RESULTS: OA challenge caused both inflammation and airway hyperreactivity, and there was a significantly positive correlation between them. Oral theophylline (1-12.5 mg/kg, bid for 7 days) attenuated antigen-induced inflammation (swelling of bronchiole walls and pulmonary eosinophilia) and bronchial hyperreactivity. CONCLUSION: These findings confirm that bronchial hyperreactivity positively correlates to airway inflammation in the rat, and suggest that theophylline at relatively lower doses has anti-inflammatory effect in airway allergic reaction.