生长抑素在类固醇糖尿病大鼠胰腺中的表达

Somatostatin expression in rat islet of steroid diabetes mellitus

目的 :探讨不同剂量地塞米松 (DEX)对大鼠胰岛细胞激素表达的影响。方法 :雄性成年SD大鼠14 0只随机平均分成 4组 ,应用放射免疫及原位杂交等技术观察不同剂量DEX作用下胰岛激素合成、储存及释放改变。结果 :在一定范围内空腹血糖 (FBG)、血清胰岛素 (Ins)与DEX给药呈剂量及时间依赖性。胰腺中生长抑素 (SS)的mRNA和蛋白表达以及血清中Ins水平的改变均早于FBG的升高。结论 :DEX改变胰岛细胞Ins和SS的表达 ,这在类固醇糖尿病 (SDM)发生过程中可能发挥重要作用。

AIM: To investigate the effects of dexamethasone(DEX) on the pancreatic endocrine function when steroid diabetes mellitus(SDM) occurred. METHODS: 140 rats were randomly assigned to 4 groups according to the clinic doses of DEX administration, Radioimmunoassay and in situ hybridization were applied to evaluate islet hormone changes in pancreas and blood.RESULTS: Changes in fasting blood glucose(FBG) ?fasting serum insulin(FINS) caused by DEX were dose and time-dependent. Changes in somatostatin(SS)mRNA and protein in islet as well as FINS occurred earlier than that of FBG. CONCLUSION: The super-physiological DEX influenced the expression SS and insulin secretion in islet, which may play an important role in SDM.