慢性乙型肝炎病人HBcAg特异性CTL的体外诱导及活性

In v itro induction and expansion of HBcAg specific CTL from chronic hepatitis B patients and cytotoxicity assays

目的从慢性乙型肝炎病人外周血单个核细胞 ( PBMC)中诱导和扩增乙型肝炎病毒核心抗原 ( HBc Ag)特异性细胞毒性 T淋巴细胞 ( CTL )。方法含乙型肝炎病毒重组逆转录病毒载体转染病人自身永生化的 B细胞作为抗原递呈细胞 ,联合重组 HBc Ag和 IL- 2 ,从病人 PBMC中诱导和扩增 HBc Ag特异性 CTL,51 Cr释放法检测其细胞毒活性。结果此方法诱导的 CTL 可以有针对性的杀伤靶细胞 ,而不能够杀伤空载体转染及未经转染的 B细胞。结论所建立的方法可以在慢性乙型肝炎病人的 PBMC中诱导出 HBc Ag特异性

ObjectiveTo induce and expand HBcAg specific CTL activity in vitro from periphera l blood monocular cells of chronic hepatitis B patients. MethodsHBcAg specific CTL was induced and expanded in vitro by EBV transformed a utologous B cells transferred with recombinant vector pXT1 HBV/C as antigen p resent cells combined with rHBcAg and IL 2. Cytotoxicity assays were performe d with 51 Chromium release assays through autologous B t ransferred pXT1 HBV/C as target cells. Result CTL induct ion can specifically kill target cel ls, but can not kill B cells transferred with wild vectors and untransferred co ntrol B cells. Conclusion HBcAg specific CT L could be induced in vitro from peripheral blood monocular cells of chronic hepatitis B patients by the method established in the article.