凋亡相关基因在肝癌及正常肝组织表达概况

Gene apoptosis expression profiles in liver cancer and their comparison to normal peri-cancerous liver tissues

目的 了解凋亡相关基因在肝癌中的表达概况 ,探寻在肝癌及正常肝组织中的表达差异。方法 以肝癌及癌旁正常肝组织的总RNA反转录合成含有α 32 PdATP的cDNA为探针 ,与Atlas微阵列表达分析膜进行差异杂交 ,并应用半定量逆转录聚合酶链反应 (RT PCR)验证。结果 放射自显影结果经AtlasImageTM软件分析显示 ,在所分析的与凋亡相关的 6 9个基因中 ,Akt1等 4个基因在肝癌组织中表达上调 ,BAK、Caspase 3等 19个基因表达下调。RT PCR结果证实了Atlas微阵列差异杂交的准确性。结论 通过Atlas微阵列系统分析发现的这些基因的表达改变 ,组成了一个肝癌特异的与凋亡相关的基因表达谱 ,全面系统地研究肝癌中与凋亡相关的基因表达改变 ,为探讨肝癌的发病机制提供了有益的线索。

Objective To describe a few gene expression profiles related to apoptosis and to demonstrate their difference between the liver cancer and its adjacent normal tissues. Methods cDNA, labeled as a 32 P dATP was synthesized from the total RNA of cancer and adjacent normal tissues and, then, hybridized individually with two identical array membranes of Atlas human cancer dDNA expression containing 588 known genes. The method involving gene specific semi quantitative reverse transcription in the polymerase chain reaction (RT PCR) was used to confirm the expression pattern of three known genes. Results Autoradiographic results were analyzed by specific AtlasImage TM (version 1.01a) software. Among 69 genes that were found to be related to apoptosis, 4 were up related, including Aktl, and 19 were down related, including BAK, Caspase 3, etc.,all being related to cancer. The RT PCR results showed that the expression pattern of all genes was in agreement with the results of Atlas expression array. Conclusion The result obtained from Atlas microarray provides a liver cancer specific expression profile related to apoptposis. The discovery about the apoptosis related genes may yield some clues on the oncogenetic mechanism of liver cancer in the future. The differential hybridization technique of cDNA expression array involving the Atlas TM human cancer can become a useful method in the understanding of the development and progression of human cancer.