双功能抗体介导胃癌杀伤效应的研究

Bispecific McAb mediated multiple tumor cytotoxicity in gastric cancer

目的 以化学方法将抗CD3 及抗三硝基苯 (TNP)的单克隆抗体 (McAb)交联为双功能抗体。利用此双功能抗体介导人外周血淋巴细胞 (PBLS)对于不同抗胃癌McAb所针对的胃癌细胞进行体内外杀伤试验。方法 利用化学交联法制备双功能抗体 ,以酶联免疫吸附试验 (ELISA)测定McAbs活性 ,以ELISA桥联法、琼脂糖免疫双扩散及十二烷基磺酸钠—聚丙烯酰胺凝胶电泳 (SDS PAGE)对于双功能抗体进行鉴定 ,以细胞杀伤试验及肿瘤生长抑制试验检测双功能抗体作用。结果 将抗CD3 及抗TNPMcAb交联为双功能抗体 ,此双功能抗体可介导PBLS对于不同抗胃癌McAb所针对的胃癌细胞进行体内外杀伤试验。结论 此双功能抗体在肿瘤生物免疫治疗中具有应用前景。

Objective McAb against CD 3 and McAb against TNP were combined into bispecific McAb by chemical methods. With this bispecific McAb, human peripheral blood lymphocytes (PBLS) were introduced to in vitro and in vivo cytotoxic test of any tumor cells by different TNP McAb against gastic cancer. Methods The activity of bispecific McAb prepared by chemical method, was detected by enzyme linked immunosorbent assay (ELISA). The bispecific McAb was identified by bridge ELISA、 Ouchterlony test and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Cytotoxic test of tumor cells in vitro and tumor growth inhibition test in vivo were performed for the bispecific McAb function. Results Anti CD 3 and Anti TNP McAbs were successfully combined into bispecific McAb which could conjugate any TNP McAb against gastic cancer and induce PBLS to cytotoxic test of gastric cancer cells in vitro and in vivo. Conclusion The bispecific McAb may very will be useful in the immuno biotherapy of tumors.