小鼠黑色素瘤某些生物学特性与其侵袭潜能的相关性研究

Correlation between the biological behavior and invasion potential in three mouse melanoma cell lines

目的 研究具有相同起源而转移能力不同的小鼠黑色素瘤细胞系 (B16、B16F10、B16BL6 )的某些生物学特性与其侵袭潜能的相关性。方法 用重组基质膜实验考察细胞的侵袭能力 ;用分光光度法测定黑色素瘤细胞的黑色素含量 ;利用细胞运动记录分析系统研究细胞在三维胶原基质中的运动状态 ;用明胶底物酶谱法分析细胞分泌Ⅳ型胶原酶的能力 ;用端粒重复扩增 (TRAP) PCR法测定细胞的端粒酶活性。结果 B16BL6和B16F10具有较高的侵袭能力 ,其运动能力及分泌Ⅳ型胶原酶的能力亦较强 ,但B16F10的黑色素含量却较低 ,端粒酶活性在三者之间无明显差异。结论在所研究的小鼠黑色素瘤不同亚系的某些生物学特性中 ,侵袭能力与细胞运动能力、分泌Ⅳ型胶原酶的能力之间有较好的相关性 ,而与黑色素含量及端粒酶活性无直接相关性。

Objective To study the correlation between biological behavior and invasion potential in three homologous mouse melanoma cell lines (B16, B16F10,B16BL6). Methods The invasion potential of melanoma cells was assayed in a Transwell cell culture chamber. Melanin content was determined by the A value (optical density) at 470 nm in spectrophotometry. Tumor cells migration within the 3 D collagen matrix was microscopically recorded with a time lapse video recorder and computer assisted cell tracking system. Gelatin zymography was adopted to assay the type Ⅳ collagenase secretion. A PCR based telomeric repeat amplification protocol (TRAP) was used for assaying telomerase activity. Results B16BL6 and B16F10 cells showed higher invasion potential than B16, but the melanin content in B16F10 was very low.B16F10 and B16BL6 possessed a higher migration ability, secreting much more type Ⅳ collagenase than the B16 though without significant difference as discovered in telomerase activity of the three melanoma cell lines. Conclusion Invasion capability of mouse melanoma is closely correlated to tumor cells migration ability and secretion of type Ⅳ collagenase. Melanin content and telomerase activity are not related to the invasion ability of mouse melanoma cells.