摘要
目的 观察经颅重复性磁刺激 (r TMS)后海马ΔFos B阳性标记的变化 .方法 SD大鼠头部予以 1Hz,10 0m T的磁刺激 (美国 Magstim公司 ) ,每日一次 ,每次 10 m in,共 14d.最后一次刺激后 2 4h,灌注动物并取脑 ,经海马冠状切片 ,免疫组织化学 ABC法检测海马各区 ΔFos B免疫阳性产物的表达 .结果 r TMS后 ,大鼠海马齿状回及 CA1区表现出明显增多及染色增强的 ΔFos B免疫阳性产物 ,CA2 ,CA3区则未出现 ΔFos B免疫阳性产物 ;假刺激组动物仅海马齿状回表现出少许淡染的 Δ Fos B免疫阳性产物 ,CA1,CA2 ,CA3区则无Δ Fos B免疫阳性产物出现 .结论 我们认为r TMS后ΔFos B免疫阳性产物在海马的集聚 ,提示 r TMS可能通过影响海马长时程神经和行为塑形而发挥其治疗作用 .
AIM To observe the effects of repetitive transcranial magnetic stimulation (rTMS) on ΔFosB expressions in rat hippocampi. METHODS SD rats were treated with repetitive 1 Hz, 100 m Tesla transcranial magnetic stimulation for 14 d, 10 minutes each day and then ΔFosB expressions in hippocampi were detected immunohistochemically. RESULTS High and deeply stained ΔFosB expressions were induced in hippocampi of experimental rats, mainly located in the granule cell layer of dentate gyrus and pyramidal cell lay er of CA1 area. No ΔFosB immunoreactive nuclei were seen in CA2 and CA3 areas. In controlled animals, few and slightly stained ΔFosB positive neurons were found in the granule cell layer of dentate gyrus, but no ΔFosB expressions appeared in CA1, CA2 and CA3 areas. CONCLUSION ΔFosB proteins could gradually accumulate in the hippocampi with repeated treatment of transcranial magnetic stimulation, which may play some important roles in treating neuropsychological diseases by mediating long lasting neural and behavioral plasticity.
出处
《第四军医大学学报》
北大核心
2001年第14期1272-1275,共4页
Journal of the Fourth Military Medical University
