不同水稻品种种子耐超干性差异及其热稳定蛋白的研究

Difference of Ultra-drying Tolerance and Heat-stable Proteins in Seeds of Different Rice Cultivars

以不同水稻品种 (籼稻、粳稻、杂交稻等 )为材料 ,研究了品种之间种子耐超干能力的差异及其与热稳定蛋白的关系。结果表明 ,供试的粳稻品种 (春江 15和春江 6 83)不耐超干 ,当含水量降低至 4.5 %以下 ,种子发芽率和活力指数极显著下降 ,而籼稻品种 (J10 6和 J174)耐超干能力强 ,含水量降低至 3.5 %以下对种子发芽率和活力指数没有影响 ,杂交稻 (汕优 6 3)和中香糯介于两者之间。耐超干的品种存在一特异性的热稳定蛋白条带 ,其分子量为 36 .3k D,而不耐超干的品种种子无此条带 。

The difference of ultra drying tolerance and the heat stable proteins were studied among different rice cultivars, and two ideas inferred from the results. (1) The germination rate and vigor index of japonica rice seeds (Chunjiang 15 and Chunjiang 683) decreased rapidly when their moisture content fell to 4.5%. On the contrary, the germination rate and vigor index of indica rice (J174 and J106) seeds changed little even when their moisture content fell to 3.5%, which indicated that indica rice had strong ultra drying tolerance. While the seeds' tolerance of waxy rice and hybrid rice (Shanyou 63) were intervened in japonica rice's and indica rice's. (2) SDS PAGE electrophoresis showed that a 36.3 kD heat stable protein appeared only in seeds of rice cultivars (indica rice, waxy rice and hybrid rice) with ultra drying tolerance. These results suggested that the special heat stable proteins might be related to ultra drying tolerance.