一氧化氮对致敏大鼠气道炎症嗜酸性粒细胞的影响

Effects of Nitric Oxide on the Airway Inflammatory Eosinophils in Sensitized Rats

为探讨内源性一氧化氮 (NO)对支气管哮喘 (哮喘 )气道炎症嗜酸性粒细胞 (EOS)的影响 ,用鸡卵清蛋白 (OVA)腹腔致敏和反复超声雾化吸入 ,刺激复制大鼠过敏性气道炎症模型 .结果表明 ,正常大鼠 (6只 )支气管粘膜下未见嗜酸性粒细胞 ,而致敏大鼠 (6只 )支气管粘膜下EOS〔2 5± 5 (平均每视野计数 ,下同 )〕增多 .NO合成抑制剂 (NOS ,4只 )可使致敏大鼠气道EOS(13± 5 ,P <0 0 5 )减少 ,而NO合成前体则有使EOS进一步增多的趋势 。

To investigate the relations between intrinsic nitric oxide(NO) and airway inflammation eosinophils in bronchial asthma,the rat model of asthmatic airway inflammation was established by first sensitizing and then challenging the animals with ovalbumin.NO synthase(NOS) inhibitor and NO precursor were then applied and their influences on the airway inflammatory eosinophils numbers were observed.The result showed that nomal control rats did not have eosinophils in their submucosal of airways while in the sensitizecl animals eosinophils〔25±5(average cell counts per microscopic visual field,the same following)〕were found in significantly increased numbers in the airways.The application of NOS inhibitor significantly reduced eosinoplils(13±5, P <0 05).On the contrary the application of no precursor resulted in the further increase of the numbers of eosinophils.