免疫组化法检测生殖道病变组织中的人乳头瘤病毒

Detection of Human Papillomavirus in Histologic Lesions of Genital tract by immuno-histochemical Methods

对宫颈炎、宫颈鳞状上皮Ⅰ—Ⅱ级不典型增生,外阴尖锐湿疣、宫颈原位癌及浸润癌共91例及男性肛门、包皮尖锐湿疣(3例)、寻常疣或扁平疣(6例)、食管癌(15例)和晚期卵巢癌(2例)用免疫过氧化物酶法(PAP法)检测组织细胞核内HPVCA。结果表明,HPVCA多见于尖锐湿疣(14/27例)、寻常疣类(4/6例)及轻中度不典型增生(6/21例)等病变组织,而且主要分布在上皮的浅表层。这些组织中也有不少挖空细胞,但两者并不完全一致。宫颈浸润癌HPVCA阳性率最低(1/17例)。卵巢癌及食管癌均呈阴性。

Ninety one cases with cervicitis(6), CIN-Ⅰ-Ⅱ grade dysplasia(23), vulval condyloma acuminata(24), cervical carcinoma in situ(21) and invasive carcinoma(17), and other 26 cases with esophagealcarcinoma(15), ovarian carcinoma(2), verruca vulgaris(6) andmale anal condyloma acuminata(3) were screened for the presenceof papillomavirus antigens by immunoperoxidase (PAP) technique,using a genus specific anti-bovine papillomavirus serurn(Dakopatts).The results showed that positive PAP staining appeared in the super-ficial layer in most lesions of condyloma acuminata(14/27), verrcavulgaris(4/6) and CIN-Ⅰ-Ⅱ(6/21), and morekoilocytes in theselesions were also observed. Positivity was lowest in invasive cervicalcarcinomas(1/17) and no viral antigen werefound in esophageal andovarian carcinomas.