期刊文献+

激光切割植物染色体的研究 被引量:4

The research to Cutting Chromosome of Plant by Laser
下载PDF
导出
摘要 文章探索了短脉冲激光与微细玻璃针相结合对黑麦染色体进行切割、分离的技术。该技术具有操作简便 ,节省经费 ,容易掌握 ,能减少染色体 DNA的损伤 ,且可对整个细胞进行分离的优点 ,有利于促进染色体或染色体片段分离技术在进行 DNA扩增 ,建立相应的 In this article,it have explored the technique to cutting and separate the target chromosomes of rye from other chromosomes of rye by short impulse laser to combine with tiny needle of glass, the technique is provided with operating easy, economizing the use of funds, having in hands easily and could be reducing the damage of DNA in the chromosomes of rye. there are advantages to separating an whole cell, it is advantageous to promoting the technique of separating the chromosomes or fragments of chromosome put in use of increasing DNA and constructing DNA library ;to promoting the technique popularizing.
出处 《云南师范大学学报(自然科学版)》 2001年第5期52-54,共3页 Journal of Yunnan Normal University:Natural Sciences Edition
基金 国家自然科学基金资助
关键词 激光 玻璃针 染色体 分离技术 切割 植物 laser needle of glass chromosome separation technique
  • 相关文献

参考文献6

二级参考文献26

  • 1夏家辉,杨毅,戴和平,潘乾,李麓芸.14个染色体区带特异性探针池的构建[J].Acta Genetica Sinica,1994,21(4):253-256. 被引量:19
  • 2宋文芹,崔香芹,许文胜,李秀兰,彭永康,陈瑞阳.蚕豆大M染色体长臂端部的显微切割与PCR扩增[J].科学通报,1996,41(4):361-363. 被引量:30
  • 3Deng H X,Hum Genet,1992年,89卷,13页
  • 4Deng H X,Hum Genet,1992年,89卷,13页
  • 5Jung C,Plant Mol Biol,1992年,20卷,503页
  • 6Dang Benyuan,Hu Zanmin,Zhou Yihua,Cui Lihua,Wang Lanlan,Li Liangcai,Chen Zhenghua. Construction of single-chromosome DNA library fromLilium regale Wilson[J] 1998,Chinese Science Bulletin(5):434~439
  • 7Andreas Houben,Ralf G. Kynast,Ute Heim,Hanno Hermann,R. Neil Jones,John W. Forster. Molecular cytogenetic characterisation of the terminal heterochromatic segment of the B-chromosome of rye (Secale cereale)[J] 1996,Chromosoma(2):97~103
  • 8Jutta Müller-Navia,Almut Nebel,Engelhardt Schleiermacher. Complete and precise characterization of marker chromosomes by application of microdissection in prenatal diagnosis[J] 1995,Human Genetics(6):661~667
  • 9Xin-Yuan Guan,Paul S. Meltzer,Ann C. Burgess,Jeffrey M. Trent. Coverage of chromosome 6 by chromosome microdissection: generation of 14 subregion-specific probes[J] 1995,Human Genetics(6):637~640
  • 10K. Arumuganathan,Gregory B. Martin,H?kan Telenius,Steven D. Tanksley,Elizabeth D. Earle. Chromosome 2-specific DNA clones from flow-sorted chromosomes of tomato[J] 1994,MGG Molecular & General Genetics(5):551~558

共引文献33

同被引文献105

引证文献4

二级引证文献30

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部