磷钼杂多酸光度法测定水果、蔬菜、药物中抗坏血酸

Determination ascrobic acid in fruits,vegetables and medicines by spectrophotometry using phosphomolybdic heteropoly acid

研究了抗坏血酸磷钼蓝的显色反应体系 ,考察测定最佳条件 ,建立了一种测定水果、蔬菜、药物中抗坏血酸含量的方法 .该法检测限为 1 .0× 1 0 - 7g/m L,抗坏血酸质量浓度 0 .0 1 0～1 .5 0 0 mg/5 0 m L范围内服从比尔定律 .已用于水果蔬菜中抗坏血酸的测定 .

A new method to determine ascrobic acid(AA) in fruits, vegetables and medicines is set up in this paper, also, the most suitable conditions of this method are evaluated. The detection limit of this method is 1.0×10 -7 g/mL. The concentration of AA varying from 0.010 to 1.500 mg/50 mL obeys the Beer Law. This method has been used to determine AA in fruits and vegetables.