猪主动脉内皮细胞培养

Porcine Aortic Endothelial Cell Culture in Vitro

本实验参照Loskutoff等牛主动脉内皮细胞培养法加以改进,成功地进行了猪主动脉内皮细胞培养。由于猪主动脉取材方便,细胞获得量大、易于培养且可繁殖传代,故是一种实用的工具细胞。本法为在体外观察血管内皮细胞的生物学、病理学、免疫学特征,以及对研究循环疾病、出凝血机制等提供了方便。

A method of porcine aortic endothelial cell culture was exploredand established in our laboratory. The convenience in the source ofporcine aortae, the abundance of cells, the facileness for subculturewithout the requirement for growth factors, porcine endothelial cul-ture would be a very useful toll for the research of biological, patho-logical, and immunological characteristics on endothelial cells, and of the circulation disease, thrombotic and fibrinolytic mechanisms aswell.