得克萨斯红末端修饰单链DNA探针微阵列ZrO_2陶瓷小珠的发光特性研究

Fluorescent Features of the ZrO_2 Ceramic Beads Microarrayed with Texas Red Terminus-Modified Single Stranded DNA Probes

以N ( 4 马来酰亚胺氧化丁酰 ) 琥珀酰亚胺磺酸钠盐 (Sulfo GMBS)为偶链剂将 5 NH2 /3 Tex双末端修饰DNA探针偶链到 3 0 0 μmZrO2 陶瓷小珠表面上 ,制成了发红光的陶瓷小珠 .理论研究表明 ,小珠表面上 2 0 mer单链DNA探针之间的分子间距和微阵列密度是偶链反应溶液中DNA探针浓度、小珠数目和小珠大小的函数 .在优化条件下 ,2 0 mer单链DNA探针在小珠表面微阵列的最小分子间距约为 8 0nm ,因而在一个 3 0 0 μmZrO2 陶瓷小珠表面有10 10 数量级的 2 0 mer单链DNA探针进行微阵列 .

By coupling 5-NH 2/3-Tex terminus modified single stranded DNA probes on the surfaces of 300 μm ZrO 2 ceramic beads through N-(4-maleimidobutyryloxy)-sulfosuccininimide (Sulfo-GMBS), fluorescent ceramic beads were prepared. Theoretical studies show that the microarray distance and density of DNA probes on the beads surface depend on the concentrations of DNA in aqueous solution, beads number and size. On optimal conditions, the distances between 20-mer single stranded DNA probes on the beads surface is near 8.0 nm, therefore more than 1×10 10 of 20-mer DNA probes were dotted on the surface of each 300 μm ZrO 2 ceramic beads.