摘要
为探讨组织多肽特异性抗原 (TPS)在肝癌的表达及其血清水平与癌细胞增殖活性的关系 ,选择 96例肝癌手术病人 ,ELISA法测定术前血清TPS水平 ,术后肝癌组织切片做角蛋白 18(CYK18)、增殖细胞核抗原 (PC NA)的免疫组化 (IHC SABC)染色 ,并比较血清TPS水平与PCNA阳性的关系。结果见所有肝癌组织切片CYK18、PCNA染色均为阳性 ,其中PCNA染色 +6 0例 (6 2 5 % ) , 2 5例 (2 6 0 % ) , 8例 (8 3% ) , 3例 (3 1% ) ;各组中位TPS血清水平随PCNA染色阳性强度的增加而增高 (P <0 0 1)。表明肝癌细胞膜上高度表达CYK18。
To investigate the expression of cytokeratin 18(CYK18) in hepatocellular carcinoma(HCC) cell membrane and the relationship between the serum levels of tissue polypeptide specific antigen (TPS) and the proliferating activity of HCC cells. The serum levels of TPS were determined by TPS TM ELISA in 96 patients with HCC. Four micrometer paraffin embeded sections of liver specimens from these patients were immunostained with the strept avidin biotin complex immunoperoxidase technique (IHC SABC). All of the HCC preparations were positively stained by CYK 18 and proliferating cell nuclear antigen (PCNA) with IHC SABC.The results of PCNA stainings were: + in 62 5%, in 26 0%, in 8 3%,and in 3 1% .The median levels of TPS in the four groups were: + 137 98 U/L, 685 3 U/L, in 1 126 U/L and 4 672U/L respectively. There were significant differences among these groups ( P <0 01,K W test). These results show that CYK 18 is highly expressed in the cell membrane of HCC, and the serum levels of TPS can reflect the proliferating activity of HCC.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2001年第11期787-789,共3页
Medical Journal of Chinese People's Liberation Army
基金
国际科技合作课题 (瑞典康乃格生物工程研究所资助
编号98C0 1)