摘要
为研究fas基因转导对食管癌细胞的体外抑制作用 ,构建fas基因真核表达载体fas pBK并将其转导入食管癌细胞EC10 9。Westernblot结果证实fas基因转导株Fas EC10 9表达高水平的Fas蛋白 ;细胞生长曲线和软琼脂集落形成实验结果显示 ,与对照细胞相比 ,Fas EC10 9的群体倍增时间延长 ,克隆形成能力降低。MTT比色法表明Fas EC10 9对CDDP、VCR、5 FU的敏感性明显增加。本研究结果提示 ,转导fas基因能有效抑制体外培养的食管癌细胞的生长 。
To evaluate the inhibitory effects of fas gene transduction on oesophageal cancer cells in vitro, the eukaryotic expression vector fas pBK was constructed and transfected into oesophageal carcinoma cells EC109. Western blot results showed high expression of Fas protein in gene transfected cells Fas EC109. Cell growth curve and plating efficiency test revealed that Fas EC109 had longer population doubling time and lower plating efficiency compared with control cells. Results of MTT assay showed increased sensitivity of Fas EC109 cells to CDDP, VCR and 5 FU. All these data suggested that transgenic expression of fas gene could effectively inhibit the proliferation and increase the drug sensitivity of oesophageal carcinoma cells in vitro.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2001年第11期801-803,共3页
Medical Journal of Chinese People's Liberation Army