阿霉素肾病组织环孢素亲合素基因的表达

THE EXPRESSION OF CYCLOPHILIN mRNA IN ADRIAMYCIN NEPHROPATHY

应用传统的方法建立阿霉素肾病模型 ,通过逆转录聚合酶链反应 (RT PCR)测定阿霉素肾病肾组织环孢素亲合素基因表达 ,及与血总蛋白、白蛋白、血胆固醇、2 4h尿蛋白定量、血尿素氮、肌酐之间的关系。结果表明 ,8只SD大鼠经阿霉素注射后 ,尿蛋白在注射后 1周开始上升 ,2周后明显增加 ,4周后尿蛋白均 >10 0mg/ 2 4h ,对照组尿蛋白始终在 6mg/ 2 4h以下。阿霉素肾病肾组织环孢素亲合素基因表达比对照组明显增高。同时发现阿霉素肾病肾组织环孢素亲合素基因表达的水平与血总蛋白和白蛋白的关系成负相关 ;与血胆固醇及 2 4h尿蛋白定量成正相关 ;与血尿素氮及肌酐之间无相关关系。结果提示 ,阿霉素肾病肾组织高水平的环孢素亲合素基因表达是肾组织损伤的原因之一 。

We have established adriamycin(ADR) nephropathy model, the expression of cyclophilin(CyP)mRNA was measured with RT PCR, and its relationship with the levels of total protein, albumin, cholesterol, 24 hour urine protein, BUN, and creatinine were determined.After four weeks,the levels of urine protein in ADR nephropathy animals were higher than 100mg/24h, whereas the levels in the control animals were lower than 6mg/24h. It was showed that the expression of CyP gene of kidney tissue in ADR nephropathy rats was significantly higher than that of the control animals. Meanwhile, it was found that there was negative correlation was found between the expression of Cyp gene and the levels of total protein and albumin,but positive correlation between the expression of CyP gene and the levels of 24 hour urine protein and cholesterol,and the expression of CyP gene had no correlation with the levels of BUN and creatinine. Our results suggested that the high levels of Cyp mRNA expression might contribute to renel tissue damage in ADR nephropathy,and it might reflect the degree of severity and prognosis in ADR nephropathy.