结、直肠癌组织中PNA、PHA及CEA的免疫组化研究

Immunohistochemical Study of Peanut Agglutinin (PNA), Phaseolus Vulgaris Agglutinin (PHA) and Carcinoembryonic Antigen in Colorectal Adenocarcinoma

用生物素标记的花生凝集素(PNA)、菜豆凝集素(PHA)及抗CEA抗体对良恶性结、直肠组织作组化染色研究,发现:PNA主要标记结、直肠癌(21/25);PHA主要与正常结、直肠杯状细胞粘液相结合(21/22);而癌旁粘膜的结合情况介于二者之间。上述凝集素能显示细胞糖结合物糖化的紊乱,因而有助于结肠癌的诊断。抗CEA抗体可帮助临床决定哪些病人需定期随访检查血中CEA水平,以便监测复发与转移。

An immunoenzymologic method has been applied to the identifi-cation of lectin-binding sites as well as carcinoembryonic antigen(CEA) on paraffin sections of benign and malignant colorectaltissues. There is an inerease of PNA receptor in malignant cell thanin normal epithelial cell, 84% (21/25) of cancer specimens expressedPNA-binding sites. Conversely, the lectin PHA avidly binds to themucin found in well-differentiated goblet cells in the normal colorec-tum, it binds to all but 1 colorectal controls (21/22). Being ableto indicate glycosylated disorder of epithelial cell glycoconjugatesin colonic mucosa, the above lectin might help diagnosis of coloniccancer. The experimental result also showed that CEA might bedeveloped into an effective probe into the surveillance of the meta-stasis and recurrence of colonic cancer.