胃粘膜细胞线粒体DNA核内整合及其与幽门螺杆菌感染的关系

Relationship between integration of mtDNA fragments in the nuclei of gastric mucosal cells and Helicobacter pylori infection

目的 探讨胃粘膜细胞核内mtDNA整合与幽门螺杆菌 (Hp)感染的关系。方法 采用PCR、免疫组化和Giemsa染色法检测Hp ;采用原位杂交方法对胃粘膜细胞核内mtDNA序列进行检测。结果 胃癌细胞核内mtDNA序列的检出率为 2 0 %、癌旁粘膜为16 .7%、异性增生为 2 0 %、肠上皮化生粘膜为 10 %、萎缩性胃炎为 10 % ,浅表性胃炎未发现有mtDNA序列整合。胃粘膜细胞核内mtDNA序列的检出率在Hp+ 组为 2 1.1%显著高于Hp-组 ( 5 .3% ) ;在cagA+ Hp感染组 ( 2 4.4% )显著高于cagA-Hp感染组 ( 7.4% )。结论 ①胃粘膜细胞mtDNA序列核内整合可能参与了胃癌的发生 ;②胃粘膜mtDNA序列整合可能与Hp感染有关。

Objective To explore the relationship between the integration of mtDNA in the nuclei of gastric mucosal cells and Helicobacter pylori infection. Methods Hp was detected by PCR,immunohistochemical method and modified Giemsa stain; the sequences of mtDNA in the nuclei was detected by in situ hybridization. Results The integration rate of mtDNA in the nuclei was 20% in gastric cancer, 16.7% in mucosa adjacent to gastric cancer lesion, 20% in dysplasia, 10% in intestinal metaplasia, 10% in chronic atrophic gastritis and none in superficial chronic gastritis. The integration rate in Hp + group(21.10%)was significantly higher than that in Hp - group(5.3%);cagA + Hp group(24.4%) was significantely higher than that in cagA - Hp group(7.4%)? Conclusion Integration of mtDNA into nuclei of gastric mucosal cells may be involved in the carcinogenesis of gastric mucosal cells and Hp infection may contribute to the integration of mtDNA into nuclei of gastric mucosal cells.