原儿茶醛对血瘀证患者外周血单个核细胞趋化游走能力变化的影响

Effects of protocatechuic aldehyde on chemotactic migration of peripheral blood mononuclear cell of patients with blood stasis syndrome

目的和方法 :观察献血员和血瘀证患者外周血单个核细胞 (PBMNC)跨越聚碳酸酯 (PC)膜及跨越人脐静脉内皮细胞 (HUVEC)单层趋化游走能力的差异、游走过程中产生IL - 8活性水平的变化及原儿茶醛 (PCA)对上述过程的影响。结果 :血瘀证患者组的趋化游走的PBMNC数目明显多于献血员组 ;PCA明显地抑制该 2组PBMNC的趋化游走。血瘀证患者PBMNC在游走过程中产生的IL - 8活性明显高于献血员 ;PCA抑制该 2组患者的PBMNC产生的IL - 8活性。结论 :血瘀证组PBMNC的趋化游走活性明显增强 ,并产生更高的IL - 8活性。PCA抑制PBMNC趋化游走和在趋化游走过程中产生的IL - 8活性。

AIM and METHODS: Chemotactic migration of peripheral blood mononuclear cell(PBMNC) of healthy blood donors(BD) and patients with blood stasis syndrome(BSS) across polycarbonate membrane(PCM) and human umbilical vein endothelial cell(HUVEC) monolayer, IL-8 produced by migrat PBMNC and effects of protocatechuic aldehyde(PCA) on the process mentioned above were investigated. RESULTS: 1) The numbers of migrating PBMNC in group BSS was higher than that in group BD(P<0.01); 2) PCA inhibited PBMNC chemotactic migration in both groups; 3)Activity of IL-8 production PBMNC in group BSS was higher than that in group BD (P<0.05); 4)PCA inhibited activity of IL-8 produced by PBMNC. CONCLUSION: Ability of chemotactic migration and IL-8 production of PBMNC was much stronger in patient with blood stasis syndrome than those in healthy blood doner. PCA could significantly inhibit chemotactic migration of PBMNC and production of IL-8 by PBMNC.