舒普深、哌拉西林钠的胶束电动毛细管色谱分析

Determination of sulperazon and piperacillin by micellar electrokinet iccapillary chromatography

目的 建立精密、快速简便地测定舒普深 (内含头孢哌酮和舒巴坦 )及注射用哌拉西林含量的方法。方法 采用胶束电动毛细管色谱法 (MECC) ,测定运行液采用 pH =7.5的 0 .0 2mol·L-1磷酸盐缓冲液 ,其中含十二烷基硫酸钠 (SDS) 11g·L-1,正丁醇 2 2mL·L-1,正庚烷 4mL·L-1。分离电压 18kV(运行电流 85～ 95 μA)。 结果 3种成分在 10～ 2 0min内得到完全分离 ,选择苯巴比妥作内标 ,各组分浓度在 0 .1～ 2g·L-1范围内浓度对内标峰面积之比呈良好线性关系。可准确测定 3种成分的含量 ,批内RSD均小于 1% (n =8) ,3种成分的加样回收率均在 94.92 %～ 10 5 .3%之间 ,RSD均小于 3.4% (n =8)。结论 用MECC同时测定头孢哌酮、舒巴坦、哌拉西林钠的含量 ,方便易行 ,结果准确可靠。

OBJECTIVE: To establish a method for the determination of the content of cefoperazone and sulbactam in sulperazon and piperacillin injection. METHOD: The complete separation of the components was achieved by micellar electrokinetic capillary chromatography (MECC) with 0.02 mol&middotL-1 pH7.5 running phosphate buffer containing SDS 11 g&middotL-1, 1-butanol 22 mL&middotL-1 and heptane 4 mL&middotL-1. The constant voltage was 18 kV (current of 85 ∼ 95 μA). RESULTS: The migration times of the individual components were between10 and 20 minutes with phenobarbital as the internal standard. Good linearity over the range of 0.1 ∼ 2 g&middotL-1 was obtained. The RSD of intra-run was less than 1% (n = 8) and the recoveries were 94.92% ∼ 105.3% with RSD less than 3.4% (n = 8). CONCLUSION: This method was accurate, simple and rapid. The quantity of each components may be determined using this method.