间质胶原酶在实验性肺高压肺血管重建中表达的动态改变

Gene expression of interstitial collagenase in pulmonary vascular remodeling of rats with pulmonary hypertension

目的 探讨间质胶原酶基因表达在肺动脉高压肺血管重建中的作用。方法 利用野百合碱(monocrotaline ,MCT)诱导的大鼠肺高压动物模型 ,经导管介入测定大鼠肺动脉平均压力 ,逆转录定量聚合酶链反应 (RT PCR)检测不同时间点大鼠肺组织间质胶原酶mRNA相对表达水平。结果 实验第三周肺动脉平均压力已明显升高 ,以第四周为最高 ,而大鼠肺组织间质胶原酶mRNA表达水平以实验第二周最为明显 ,第三、四周表达水平下调 ,但仍高于对照组。结论 MMP 1基因表达异常参与了肺高压肺血管重建过程 ,其早期基因过度表达有助于细胞外基质屏障的降解 ,对肺血管重建可能起了触发作用。

Objective To explore the etiology of pulmonary hypertention with relation to the expression of intersitial collagenase in pulmonary tissue. Methods The pulmonary hypertension animal models were induced by the monocrotaline (MCT); the mean pulmonary arterial pressure was measured by catheterization; and the pulmonary intersitial collagenase mRNA expression was studied by RT PCR. Results The mean pulmonary arterial pressure was increased significantly in the 3rd week after the induction of moncrotaline and reached the peak in the 4 th weak; while the pulmonary interstitial collagenase mRNA expression was increased significantly in the second week and then decreased greatly in the 3rd and 4th but still higher than that in the 1st weak. Conclusion Abnormal interstitial collagenaes expression might take part in the remodeling pulmonary hypertension; early interstitial collagenase expression might facilitate the degradation of extracellular matrix barrier, thus triggering the pulmonary vascular remodeling process.