壬基酚对仔鼠雄性生殖系统的影响

Adverse effects of exposure to p-nonylphenol on reproductive system of young male rats

目的 探讨宫内及哺乳期暴露壬基酚对雄性仔鼠生殖系统的影响。方法 对 2 8只受孕母鼠从受孕第 1天开始灌胃染毒壬基酚 (分别为 0、5 0、10 0和 2 0 0mg kg体重 ) ,直至仔鼠娩出 2 1d断奶后停止染毒 ,仔鼠于 70日龄剖杀 ,测定与仔鼠生殖功能相关的各项指标。结果 随着壬基酚染毒剂量的增加 ,70日龄雄性仔鼠的睾丸和前列腺的重量降低 ,睾丸重分别为 2 86、2 98、2 5 9和2 44g ;前列腺重分别为 0 2 6、0 2 3、0 2 0和 0 19g。每克睾丸日产精子数和每克附睾精子计数也随壬基酚剂量的增加而降低 ,每克睾丸日产精子数分别为 2 2 46× 10 6、18 46× 10 6、17 43× 10 6和 17 2 6×10 6;附睾尾精子计数分别为 46 85× 10 6、39 74× 10 6、35 5 7× 10 6和 31 36× 10 6。雄性仔鼠包皮分离时间在高剂量组 (4 7 83d)大于对照组 (4 6 31d)。结论 宫内及哺乳期暴露于壬基酚可使雄性仔鼠睾丸生精功能降低 ,但睾丸无形态学变化。

Objective To study the adverse effects of exposure to p-nonylphenol (NP) during gestation and lactation on the reproductive system of young male rats.Methods Pregnant rats were administrated with p-nonylphenol, at doses of 0, 50, 100和200 mg/kg of body weight, respectively, by gavage from the first day of gestation to the day of weaning, i.e., the 21 st day after birth. The young male rats were killed at the age of 70 days and some indices related to their reproductive system (i.e., weight of the gonad, sperm counts of the epididymis, histology of the testes, et al.) were analyzed.Result Weights of the testes and prostate gland decreased with the increase doses of exposed NP, their weights of the testes of 2.86, 2.98, 2.59 and 2.44 g, and those of the prostrate gland of 0.26, 0.23, 0.20 and 0.19 g, respectively. The same trend existed in daily production of sperm in the testes and sperm counts of the epididymis, with 22.46, 18.46, 17.43 and 17.26 ×10 6 sperms per gram of the testis, with sperm counts of 46.85, 39.74, 35.57 and 31.36×10 6 per gram of the epididymis tail. Separation of prepuce occurred on the 47.83th days and 46.31th days in the rats exposed to 200 mg/kg of NP and in the control group, respectively.Conclusion NP could decrease their ability of spermatogenesis in the rats without morphological changes of spermatogenic cells of the testes and epididymis.