摘要
目的 :探索东亚钳蝎蝎毒 (scorpionofButhusmartensiivenom)的分析及鉴定方法 ,建立相关质量标准。方法 :采用高效液相色谱法 (highperformanceliquidchromatography ,HPLC)分析检测 8个蝎毒样品。结果 :蝎毒可分离出40多个组分 ,实验所使用的 8个省内所产蝎毒具有非常相似的图谱。蝎淋巴液和 3种蛇毒与蝎毒图谱有显著不同。同一样品 (二次冻干粉 )连续测定 5次进样量的精密度、重现性均达到有关规定。结论 :采用HPLC法获得了蝎毒的指纹图谱 ,并测定了可影响蝎毒质量的蝎淋巴液和可能用于掺伪的 3种蛇毒的图谱。本研究为蝎毒及其制剂的进一步开发应用提供了有价值的实验依据。
Aim: To explore the methods on analysis and evaluation of scorpion of Buthus martensii venom(SBMV) , and set up the quality standard of SBMV. Methods:Eight samples of SBMV were inspected by high performance liquid chromatography(HPLC). Results: SBMV could be separated into more than 40 components by HPLC. The fingerprint chromatograms of 8 SBMV samples were similar, but significantly different from lymph fluid of scorpion and snake venom. The precision and reduplication of chromatogram of a single SBMV sample was also proved. Conclusion:The fingerprint chromatogram of SBMV has been set up with HPLC,and those of the lymph fluid of scorpion and 3 kinds of snake venom have been detected with HPLC in order to prevent counterfeit.The experimental results are thought as an important evidence for making use of SBMV and it's components.
出处
《河南医科大学学报》
北大核心
2001年第6期661-663,共3页
Journal of Henan Medical University
基金
河南省科技攻关资助项目 95 12 0 0 10 2
关键词
东亚钳蝎蝎毒
高效液相色谱法
指纹图谱
质量标准
scorpion venom
high performance liquid chromatography
fingerprint chromatogram