兔脑出血灶周围脑组织GFAP及S-100的表达

Expressions of GFAP and S-100 in perihematomal brain regions of rabbits

目的 :观察实验性兔脑出血灶周围脑组织GFAP和S 10 0的变化 ,探讨脑出血继发性损害的机制。方法 :家兔 36只 ,分A、B、C、D 4组 ,B、C、D 3组采用自体血注射法制作脑出血模型 ,并分别于第 3d、7d、14d将兔处死。取出血周围脑组织作切片 ,用免疫组化法检测其GFAP和S 10 0的表达。结果 :A组 (对照组 )GFAP、S 10 0表达水平均较低 ,B、C、D组表达明显增高 (P <0 .0 5 ) ,且B、C、D 3组间比较差异亦有显著性 (P <0 .0 5 )。结论 :出血灶周围脑组织GFAP和S 10 0表达水平变化既是细胞组织损伤的表现 。

Aim: To observe the expressions of GFAP and S 100 in the perihematomal brain regions and explore the pathological mechanism of intracerebral hemorrhage. Methods: A total of 36 rabbits were divided into group A,B,C and D.In group B,C and D,rabbit models of intracerebral hemorrhage were made by injection of self body blood into the cerebrums,and the animals were killed respectively on the 3 rd, 7 th and 14 th d after injection.Group A was as control.The perihematomal brain tissues were taken out. The expressions of GFAP and S 100 were tested by immnohistochemical method. Results: Expression levels of GFAP and S 100 in gliocytes of the control group were lower than those of the experiment groups( P< 0.05),among which the differences were significant ( P< 0.05). Conclusion: Changes of the expressions of GFAP and S 100 after the intracerebral hemorrhage may be the damage or repairation of the nerve tissues.