实验性变应性鼻炎鼻粘膜神经生长因子的测定

Measurement of nerve growth factor in the nasal mucosa of experimental allergic rhinitis

目的 探讨神经生长因子 (nervegrowthfactor,NGF)在变应性鼻炎发病中的作用。方法建立SD大鼠变应性鼻炎的模型 ,利用酶联免疫吸附测定 (enzyme linkedimmunosorbentassay,ELISA)法测定实验性变应性鼻炎鼻粘膜组织中的神经生长因子的含量。结果 实验性变应性鼻炎鼻粘膜的神经生长因子含量 ( x±s,下同 ;n =9)为 (6 .6 5± 0 .42 )ng/g湿重 ,对照组 (n =6 )为 (3.5 1± 0 .2 9)ng/g湿重 ,二者相比差异有显著性 (P <0 .0 0 1)。结论 神经生长因子可能通过调节神经肽的表达和释放和/或者通过活化免疫细胞 (如肥大细胞 )

Objective To investigate the role of nerve growth factor in the development of allergic rhinitis. Methods Animal model of allergic rhinitis was established in rat, the content of nerve growth factor in the nasal mucosa was measured using enzyme linked immunosorbent assay (ELISA) technique. Results The content of nerve growth factor significantly ( P <0.001) increased in the nasal mucosa of experimental allergic rhinitis (6.65±0.42) ng/g wet weight, compared with that in control animals (3.51±0.29) ng/g wet weight. Conclusion Nerve growth factor may play an important role in the development of allergic rhinitis by regulating expression and release of neuropeptides and /or by activating immune cells such as mast cells.