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鼻息肉中诱发型一氧化氮合酶mRNA的检测及其意义 被引量:4

Expression of the inducible isoform of nitric oxide synthase mRNA and the role in nasal polyps
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摘要 目的 检测鼻息肉组织和鼾症下鼻甲粘膜组织中的诱发型一氧化氮合酶 (inducibleisformofnitricoxidesynthase ,iNOS)mRNA水平的表达 ,探讨其在慢性炎症过程中的作用。方法 取 8例鼻息肉切除术的鼻息肉标本和 6例行下鼻甲切除术的下鼻甲粘膜 ,用半定量的反转录 聚合酶链反应 (reversetranscription polymerasechainreaction ,RT PCR)方法检测iNOS的mRNA表达。结果 RT PCR结果显示 8例鼻息肉中iNOS的表达 (0 .92 3± 0 .184)较 6例下鼻甲组织 (0 .15 8± 0 .2 47)明显升高 ,t检验 ,P <0 .0 1,差异有显著性意义。结论 鼻息肉组织中iNOS的表达显著升高 ,推测iNOS和一氧化氮(nitricoxide ,NO) Objective To explore the pathogenesis of nasal polyps. The aim of this study is to detect the expression of inducible isoform of nitric oxide synthase (iNOS) in nasal polyp tissue, and to compare these findings with that in normal nasal turbinate. Methods We examined the expression of iNOS in human nasal polyps from patients undergoing nasal polypectomy ( n =8) and nasal turbinectomy( n =6). The iNOS mRNA expression was determined by semi quantitative reverse transcription polymerase chain reaction (RT PCR). Results The iNOS mRNA expression was significantly stronger in the nasal polyps (NP) than that in turbinate tissues ( P <0.01). Conclusions iNOS expression is upregulated in NP, which indicate that iNOS and NO may play a potential role in the formation and growth of NP.
出处 《中华耳鼻咽喉科杂志》 CAS CSCD 北大核心 2001年第4期298-300,共3页 Chinese Journal of Otorhinolaryngology
关键词 鼻息肉 一氧化氮 一氧化氮合酶 聚合酶链反应 MRNA Nasal polyps Nitric oxide Nitric oxide synthase Polymerase chain reaction
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参考文献1

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同被引文献24

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