胃粘膜幽门螺杆菌感染与线粒体DNA微卫星不稳的关系

The relationship between Helicobacter pylori infection and mitochondrial DNA microsatellite instability in gastric mucosa

目的 探讨胃粘膜幽门螺杆菌 (Hp)感染与线粒体DNA微卫星不稳 (mtMSI)的关系。方法 采用改良Giemsa染色、免疫组化染色和PCR方法检测Hp ,并对Hp进行分型 ;采用限制性片段多态性 (PCR SSCP)方法检测胃粘膜细胞mtMSI。结果 30例胃癌检出mtMSI 11例 ( 36 .7% ) ,15例肠化中有 4例 ( 2 6 .7% )检出mtMSI ,10例异型增生胃粘膜有 3例 ( 30 % )检出mtMSI。mtMSI主要发生于D loop闭环区。Hp感染胃粘膜mtMSI发生率显著高于非Hp感染组 (P <0 .0 1)。mtMSI发生与cagA+ Hp似乎没有密切关系(P >0 .0 5 )。结论 Hp感染可能与胃粘膜细胞mtMSI发生有关 ,而mtMSI的发生可能参与了胃癌的发生过程。

Objective To evaluate the relationship between Helicobacter pylori infection and mitochondrial microsatellite instability (mtMSI) in gastric mucosa. Methods Hp was detected by modified Giemsa stain and immunohistochemical technique and Hp cagA classification was identified by PCR method; mtMSI was detected by PCR SSCP. Results The mtMSI was detected in 11 of 30(36.7%) in gastric cancers, 4 of 15(26.7%) in intestinal metaplasia, and 3 of 10 (30.0%) in dysplasia. mtMSI was mainly detected in mitochondrial DNA D loop. The mtMSI phenotype was associated with Hp infection( P <0.0), however, it was not associated with Hp cagA status ( P >0.05). Conclusion Hp infection seems to be an important factor in inducing mtMSI in gastric mucosa, and mtMSI might play a role in the carcinogenesis of gastric cancer.