摘要
1986年,在海南岛发生登革热爆发流行期间,应用型特异性单克隆抗体以间接免疫荧光法快速鉴定所分离的35株登革热毒。接种标本的第一代C_6/36细胞于感染病毒后96小时内登革病毒抗原检出率可达97％
With the microcullured by C_6/36 clone of Aedes Albopictus cells, 35 dengue virus strain were isolated from patients with dengue fever in Hainan island. These virus were identified as type 2 virus by means of four dengue virus type-specific monoclonal antibody in an indirect immunofluorescene assay. The virus antigen can be detected from postinfectious of C_6/36 cells within 48hr.as a result of using dengue type-specific McAb.
关键词
登革病毒
单克隆抗体
特异性型
Dengue fever
Type-specific monoclonal antibody
Type 2 dengue virus
indirect immunofluorescene assay
