摘要
目的 :建立确定胸腺素α1纯度、分子量和等电点等生化特性和生物学活性检测的方法。方法 :应用高效液相色谱、三羟甲基甘氨酸 -SDS -聚丙烯酰胺凝胶电泳、等电聚焦 -聚丙烯酰胺凝胶电泳和3H -TdR掺入法 ,在致有丝分裂原存在的条件下 ,刺激小鼠脾淋巴细胞分裂增殖的能力。结果 :胸腺素α1的纯度为 98% ,其相对分子质量为 3 15 3 ,等电点为3 93,在 1 5 6～ 12 5 μg·mL-1剂量范围内脾淋巴细胞的增殖率明显提高。结论 :本文所用检测方法能准确地测定胸腺素α1的纯度、相对分子质量和等电点 ,有效地测定其生物学活性 。
Objective:To identify the quality of thymosin α 1(Tα 1),some biochemical characteristics such as purity,molecular weight(\%M\% r),isoelectric point( pI )and its biological activity were studied.Methods:High performance liquid chromatography(HPLC),Tricine-SDS-polyacrylamide gel electrophoresis(Tricine-SDS-PAGE),isoelectric focusing-polyacrylamide gel electrophoresis(IEF-PAGE) and 3H-TdR incorparation methods were developed to determine its purity,\%M\%\-r,\%pI\% and the proliferative respone of the mitogen ConA stimulated spleen lymphocytes respectively.Results:The purity of Tα 1 is about 98% and it has a molecular weight of 3 153 and a \%pI\% of 3 93.Furthermore,Tα 1 has biological activity to increase the proliferative respone of the mitogen ConA stimulated spleen lymphocytes of Balb/C mice.Conclusion:These methods are simple,rapid,reliable and stable to identify the quality of Tα 1.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2001年第4期237-240,共4页
Chinese Journal of Pharmaceutical Analysis
