不同温度对压电传感器基因杂交效应的影响

Effects of temperature on hybridization efficiency in piezoelectric gene sensor arrays

目的 探讨温度对压电传感器基因杂交效应的影响。方法 在压电传感器阵列上固定 2 0碱基的巯基DNA探针 ,而后分别在 1 5℃、2 0℃、2 5℃、3 0℃下与同长度的互补靶序列进行杂交 ,计算机采集并分析传感器频率数据 ,比较各温度下杂交达到平衡所需时间 ,杂交前后频率差 ,并计算杂交动力学参数。结果 随温度增加 ,杂交时间有缩短趋势 ,杂交前后的频率差值逐渐减小。结合常数变化不明显 ,解离常数增大 ,使平衡常数显著增大。结论 在石英晶体传感器阵列上 ,基因杂交量随温度增高而减小。

Objective To investigate the effects of temperature on hybridization efficiency in piezoelectric gene sensor arrays.Methods Gene sensor arrays of 2×5 were made,gene probes of 20 bases were immobilized on Au surfaces,then hybridized with its complementary sequences at 15℃、20℃、25℃、30℃,Data of frequency changing was collected and analyzed.Comparing hybridization time and frequency differences caused by hybridization under different temperature,comparing the kinetic parameters of hybridization.Results Following the increasing of temperature,hybridization time had a trend of shortening,frequency differences caused by hybridization were decreased,and association constants were raised which due to the dissociation constants increased.Conclusion In the piezoelectric gene sensor arrays,following the increasing of temperature,hybiridization amount decreased.