小儿脑瘤中CD_(44)基因mRNA内含子9的潴留初探

Exploration for the retention of 9th intron of CD_(44) in children's cerebroma

目的 探讨CD4 4基因mRNA中内含子 9在小儿脑瘤中的潴留情况及其肿瘤学意义。方法 采用组织选择性方法提取标本RNA ,应用逆转录 聚合酶链反应 (RT PCR)技术检测 18例小儿恶性脑膜瘤 ,16例脑癌旁组织标本及脑胶质瘤 7例中CD4 4基因mRNA内含子 9的潴留情况。结果 18例恶性脑膜瘤中有 16例CD4 4内含子 9含量高 ,其中 10例表达明显 (88.9% ) ;16例脑癌旁组织只有 1例内含子潴留 ,15例无潴留现象 (6 .2 5 % ) ;脑胶质瘤 7例有 5例CD4 4基因mRNA内含子 9表达阳性 (71.4% )。恶性脑瘤中含量高于脑癌旁组织 (P <0 .0 1) ,内含子 9的潴留与小儿恶性脑瘤的组织学类型无明显关系。结论 小儿恶性脑瘤组织中存在内含子 9的潴留现象 ,而脑癌旁组织基本无潴留现象。CD4 4基因mRNA中内含子

Objective To explore the expression and significance of the remaining 9th intron of CD 44 in children's cerebroma.Methods RNA was extracted from samples with tissue selective method, and the mRNA of intron 9 of CD 44 was detected in 18 malignant meningeal tumors, 16 para tumor tissue samples, 7 brain glioma by reverse transcription polymerase chain reaction (RT PCR). Results Intron 9 of CD 44 were expressed in 16 malignant meningeal tumors( 88.89 %), among which 10 expressed obviously; only 1 para tumor tissue had intron 9 retention ( 6.25 %); 5 brain glioma expressed intron 9 positively( 71.4 %). The positive rate of intron 9 in malignant cerebroma was higher than in para tumor tissue( P < 0.01 ).Conclusion The expression of 9th intron may be related to the pathogenesis of children's cerebroma.