卵巢上皮癌组织中HLA抗原和CD80的表达

Expression and clinical significance of HLA antigen and CD80 in ovarian epithelial carcinoma

目的 了解卵巢上皮癌 (EOC)中HLA、CD80的表达及其临床意义。方法 应用免疫组化法检测HLAⅠ和HLAⅡ的表达 ,用RT PCR检测CD80mRNA的表达。结果 44份EOC中 ,HLAⅠ、HLAⅡ的阳性表达率分别为 5 9 0 9%和 43 18% ;CD80mRNA的表达率为 9 0 9%。HLAⅠ类分子的阳性表达在Ⅲ～Ⅳ期显著低于Ⅰ～Ⅱ期 (P <0 0 5 ) ,有淋巴结转移组显著低于无淋巴结转移组 (P <0 0 5 ) ,肿瘤复发组显著低于未复发组 (P <0 0 1)。HLAⅡ类分子的表达在有淋巴结转移组显著低于无淋巴结转移组 (P <0 0 5 ) ,HLAⅡ表达组较不表达组复发率有降低趋势 ,但差异未达显著水平 (P =0 0 74)。CD80mRNA阳性者 4例均未复发 ,而CD80mRNA阴性者复发率为5 7 5 % ,两组有显著性差异 (P <0 0 5 )。HLAⅠ与HLAⅡ、HLAⅠ与CD80同时缺乏时 ,患者复发率分别较HLAⅠ与HLAⅡ、HLAⅠ与CD80共表达时显著升高 (P <0 0 1)。结论 EOC可能通过HLAⅠ的降调、CD80的缺乏多种途径逃避机体的免疫监视 ,检测上述调节分子的表达 ,可能对判断患者预后及指导免疫治疗有一定的意义。

Objective To determine expressions of HLA Ⅰ,HLA Ⅱ and CD80 in tissues of epithelial ovarian carcinomas(EOC) and the clinical significance.Methods Expressions of HLA Ⅰ and HLA Ⅱ were detected by immunohistochemical technique.Expression of CD80 mRNA was examined by reverse transcriptions-polymerase chain reaction(RT-PCR).Results The positive rates of HLA I and HLA Ⅱ were 59.09% and 43.18% respectively.CD80 mRNA was expressed on 9.09% of all 44 EOC tissuses.HLA Ⅰ expression rate in stage Ⅲ～Ⅳ was lower than that in stage Ⅰ～Ⅱ;in tumors of node-positive patients was lower than that of node-negative patients.In patients with tumors expressing HLA Ⅰ antigens,recurrence rate was lower than that in patients with tumors deficient in HLA class Ⅰ(P<0.01).Expression of HLA class Ⅱ molecule in tumors with metastatic lymph node was lower than that of tumors without lymph node involvement(P<0.05).There was a trend of lower relapse rate in patients with tumors expression HLA Ⅱ than that in patients with tumors lack of HLA Ⅱ,but the difference did not reach significant level(P=0.074).In four patients with tumors expressing CD80 mRNA,recurrence didn′t occur,in contrast to patients with tumors lacking CD80 mRNA,in whom tumor relapse occurred in 57.5%(P<0.05).In patients with tumors of both HLA Ⅰ and HLA Ⅱ deficiency,relapse rate was higher than that in patients with tumous coexpression of the two molecules(P<0.05).If tumors were deficient in expressions of HLA Ⅰ and CD80,relapse rate was also higher than that of coexpression of HLA Ⅰ and CD80.Conclusion EOC cells may escape from the immune survilance of the host through downregulating expression of HLA Ⅰ,lacking CD80.Evaluation of expressions of these surface immunoregulatory molecules may be helpful for judging prognoses of EOC patients and guiding immunotherapy.