毛细管电泳法测定桑叶中的黄酮类成分──芦丁和槲皮素

Determination of Rutin and Quercetin in Mulberry Leaves by High Performance Capillary Electrophoresis

采用高效毛细管电泳法分离测定了新疆不同地区、不同采集期、不同品种的桑叶中的黄酮类成分芦丁、槲皮素的含量。以含有体积分数为 15 %甲醇的 10mmol/L的磷酸二氢钠 2 0mmol/L的硼砂溶液 (pH 8 6 2 )为电泳缓冲液 ,采用压力进样方式 ,在 2 5℃ ,2 0kV恒压下进行电泳分离 ,并在 2 45nm波长处检测。结果表明 ,桑叶中的两种目标组分在 12min内完全分离 ,且有良好的线性关系 ;芦丁和槲皮素的加样回收率分别为 95 6 4%和99 36 % ,其RSD分别为 2 2 5 %和 1 79% (n =6 )。方法简单、准确、快速。

Rutin and quercetin are the main effective components of mulberry leaves with the functions of controlling the increase of fat in serum and controlling the formation of arterio sclerosis In this article a high performance capillary electrophoretic(HPCE) method was used to separate and determine rutin and quercetin in mulberry leaves collected from different periods, different places and different kinds in Xinjiang Electrophoretic conditions were as follows: a capillary tube (75 μm i d ×57 cm(effective length,50 cm)), with 10 mmol/L dihydrogen sodium phosphate 20 mmol/L sodium borate containing 15% methanol as the running buffer (pH 8 62) and an applied voltage of 20 kV, at 25 ℃, detected at a wavelength of 245 nm Under the optimum conditions, rutin and quercetin were separated successfully from other components within 12 minutes The corrected peak areas of rutin and quercetin increased linearly with the increase of their concentrations in the range of 4 4 mg/L 28 8 mg/L and 1 8 mg/L 145 8 mg/L respectively The corresponding regression equations for rutin and quercetin were Y=71 60ρ+30 51 (r =0 999?1 ) and Y=315 17ρ-929 70 (r =0 994?9)respectively, and the recoveries were 95.64% and 99.36% The analytical results demonstrate the method is simple, quick and well reproducible, and can be used as a reliable tool for the quality control of mulberry leaves