高效液相色谱法测定柳树皮提取物中的水杨甙

Determination of Salicin in Extract of Willow Bark by High Performance Liquid Chromatography

建立了一种测定柳树皮提取物中水杨甙的反相高效液相色谱法。该法采用KromasilC18(4 6mmi.d .× 2 5 0mm ,5 μm)色谱柱 ,以甲醇 0 0 1mol/LKH2 PO4 缓冲液 (pH 4 0 1) (体积比为 15∶85 )的混合溶液作流动相 ,流速为1 0mL/min ,紫外检测波长为 2 6 5nm ,灵敏度为 0 0 4AUFS。在 8 89mg/L～ 2 84 40mg/L的范围内 ,水杨甙的峰面积Y与其质量浓度X的线性关系良好 ,回归方程为Y =- 2 91 5 2 6 0 +5 0 0 0 0 34X(r =0 9998)。水杨甙的平均回收率为 96 1%～ 10 1 2 % (n =5 ) ,相对标准偏差 (RSD)为 1 43%。该法操作简便、快速、准确。

An HPLC method for the determination of salicin in extract of willow bark is described Chromatographic analysis was carried out on a Kromasil C 18 ,5 μm column(4 6 mm i.d.×250 mm) with methanol 0 01 mol/L KH 2PO 4 buffer (pH 4 01)(15∶85, volume ratio) as mobile phase The detection wavelength was 265 nm Salicin was extracted from samples with methanol water(50∶50, volume ratio), and centrifuged Ten μL of supernatant were injected. The average recoveries were from 96 1% to 101 2% ( n =5),and the relative standard deviation (RSD) was 1 43% The method is simple, rapid and accurate